A new mammalian universal primer for the mitochondrial cytochrome b locus

Authors

  • Paula Ferracioli Programa de Pós-graduação em Zoologia, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro; Setor de Mastozoologia, Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro, Brazil
  • Marcione B. de Oliveira Setor de Mastozoologia, Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro, Brazil
  • Adrielle M. Cezar Setor de Mastozoologia, Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro; Programa de Pós-Graduação em Biodiversidade e Biologia Evolutiva, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil
  • Ana Lazar Setor de Mastozoologia, Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro, Brazil
  • Cintia Povill Setor de Mastozoologia, Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro, Brazil
  • Maíra Laeta Setor de Mastozoologia, Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro; Laboratório de Diagnósticos por DNA (LDD) & Programa de Pós-Gradução em Biociências, Universidade do Estado do Rio de Janeiro (UERJ) Rio de Janeiro, Brazil
  • Maria Clara Santos Ribeiro Programa de Pós-Graduação em Genética, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil
  • Stéphanie de Souza Cardim Setor de Mastozoologia, Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro, Brazil
  • Marcelo Weksler Setor de Mastozoologia, Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro, Brazil

DOI:

https://doi.org/10.32673/bjm.vie92.102

Keywords:

Amplification, Mammalia, Mod-L14724, mtDNA, PCR

Abstract

We designed a set of amplification primers to target the mitochondrial cytochrome b gene of the rodent genus Oligoryzomys. Combinations of two sets of primers successfully amplified complete mitochondrial and partial cytochrome b (MT-CYB), from not only Oligoryzomys, but also other mammalian species from the orders Rodentia, Didelphimorphia, Primates, Chiroptera, Cetacea, Carnivora, and Lagomorpha. Preliminary sequencing analyses revealed that these primers generate informative sequences for several mammalian species important for phylogenetic and species delimitation analyses.

Author Biographies

Paula Ferracioli, Programa de Pós-graduação em Zoologia, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro; Setor de Mastozoologia, Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro, Brazil

ORCID: https://orcid.org/0000-0003-2876-5358

Marcione B. de Oliveira, Setor de Mastozoologia, Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro, Brazil

ORCID: https://orcid.org/0000-0003-1628-3458

Adrielle M. Cezar, Setor de Mastozoologia, Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro; Programa de Pós-Graduação em Biodiversidade e Biologia Evolutiva, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil

ORCID: https://orcid.org/0000-0002-9299-0894

Ana Lazar, Setor de Mastozoologia, Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro, Brazil

ORCID: https://orcid.org/0000-0001-6515-6792

Cintia Povill, Setor de Mastozoologia, Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro, Brazil

ORCID: https://orcid.org/0000-0001-8494-8939

Maíra Laeta, Setor de Mastozoologia, Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro; Laboratório de Diagnósticos por DNA (LDD) & Programa de Pós-Gradução em Biociências, Universidade do Estado do Rio de Janeiro (UERJ) Rio de Janeiro, Brazil

ORCID: https://orcid.org/0000-0002-9210-8689

Maria Clara Santos Ribeiro, Programa de Pós-Graduação em Genética, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil

ORCID: https://orcid.org/0000-0003-2904-080X

Stéphanie de Souza Cardim, Setor de Mastozoologia, Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro, Brazil

ORCID: https://orcid.org/0009-0008-1890-6085

Marcelo Weksler, Setor de Mastozoologia, Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro (MN-UFRJ), Rio de Janeiro, Brazil

ORCID: https://orcid.org/0000-0001-8111-4779

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Published

2023-11-15

How to Cite

Ferracioli, P., Oliveira, M. B. de, Cezar, A. M., Lazar, A., Povill, C., Laeta, M., Ribeiro, M. C. S., Cardim, S. de S., & Weksler, M. (2023). A new mammalian universal primer for the mitochondrial cytochrome b locus. Brazilian Journal of Mammalogy, (e92), e922023102. https://doi.org/10.32673/bjm.vie92.102