Um novo iniciador universal para o gene mitocondrial citocromo b de mamíferos
DOI:
https://doi.org/10.32673/bjm.vie92.102Palavras-chave:
Amplificação, Mammalia, Mod-L14724, mtDNA, PCRResumo
Desenhamos um conjunto de primers de amplificação para atingir o gene mitocondrial citocromo b do gênero de roedores Oligoryzomys. Combinações de dois conjuntos de primers amplificaram com sucesso mitocondrial completo e citocromo b parcial (MT-CYB), não apenas de Oligoryzomys, mas também de outras espécies de mamíferos das ordens Rodentia, Didelphimorphia, Primates, Chiroptera, Cetacea, Carnivora e Lagomorpha. Análises preliminares de sequenciamento revelaram que estes primers geram sequências informativas para várias espécies de mamíferos importantes para análises filogenéticas e de delimitação de espécies.
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